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AFS Standard Fish Sampling Techniques and Environmental DNA (eDNA) for Characterizing Fish Relative Abundance, Biomass, and Species Composition in Small Standing Waters (< 23 ha)

Duration

September 2013 - July 2018

Narrative

Recently, examination of deoxyribiose nucleic acids in water samples (environmental DNA or eDNA) has shown promise for identifying fish species present in water bodies. In water, eDNA is the result of bodily secretions such as mucus, gametes, and feces. We investigated whether eDNA can be effective for characterizing fish relative abundance, biomass, and species composition in small (<200 ha) waterbodies. We compared fish relative abundance, biomass measured through eDNA methods and established American Fisheries Society (AFS) standard sampling methods in a subset of small Arizona lakes. We found no relationship between relative abundance and biomass of these fish captured by established methods and their DNA copies at individual sites A thesis was finished on this work in May, 2016. Partners included AZGFD.

Research Products and Activities

Thesis

  • Perez, C. R. 2016. Relationship between American Fisheries Society standard fish sampling techniques and environmental DNA (eDNA) for characterizing fish presence, relative abundance, biomass and species composition in Arizona standing waters. Master's Thesis, University of Arizona, Tucson.

Presentations

  • Perez, C. R., S. A. Bonar, J. J. Amberg, C. Rees, W. T. Stewart, C. Gill, C. Cantrell. 2015. Correlation of eDNA (Environmental DNA) surveys with traditional fish sampling surveys in standing waters. The Joint Annual Meeting of the Arizona/New Mexico American Fisheries Society and the Wildlife Society, Las Cruces, New Mexico. February 5 – February 7, 2015. Contributed Oral Presentation.
  • Perez, C. R., S. A. Bonar, W. T. Stewart, C. Gill, and C. Cantrell. 2015. Comparison of length frequency, condition, and growth of select sport fish species in Arizona with those in other areas of North America. The Joint Annual Meeting of the Arizona/New Mexico American Fisheries Society and the Wildlife Society, Las Cruces, New Mexico. February 5 – February 7, 2015. Poster Presentation.
  • Perez C., S. A. Bonar, J. J. Amberg, C. Rees, B. Ladell, B. T. Stewart, C. Gill, and C. Cantrell. 2015. Standard Fish Sampling Techniques and Environmental DNA (eDNA) As Tools to Characterize Fish Presence and Relative Abundance in Arizona Standing Waters. 145th Annual Meeting of the American Fisheries Society, Portland, Oregon, August 16-20.
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Current Staff

Federal Staff: 2

Masters Students: 9

Phd Students: 1

Post Docs: 1

University Staff: 43

5 Year Summary

Students graduated: 21

Scientific Publications: 48

Presentations: 95

 

Personnel

  • Scott BonarPrincipal Investigator
  • Christina PerezStudent

Funding Agencies

  • AZ Game and Fish

Links

Arizona Cooperative Fish and Wildlife Research Unit Cooperators

  1. Arizona Game Fish Department
  2. U.S. Fish and Wildlife Service
  3. U.S. Geological Survey
  4. University of Arizona
  5. Wildlife Management Institute