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Connon, R.E., L.S. D’Abronzo, N.J. Hostetter, A Javidmehr, D.D. Roby, A.F. Evans, F.J. Loge, and I. Werner. 2012. Transcription profiling in environmental diagnostics: Health assessments in Columbia River basin steelhead (Oncorhynchus mykiss). Environmental Science and Technology 46:6081-6087.

Abstract

ABSTRACT: The health condition of out-migrating juvenile salmonids can influence migration success. Physical damage, pathogenic infection, contaminant exposure, and immune system
status can affect survival probability. The present study is part of a wider investigation of out-migration success in juvenile steelhead
(Oncorhynchus mykiss) and focuses on the application of molecular profiling to assess sublethal effects of environmental stressors in
field-collected fish. We used a suite of genes in O. mykiss to
specifically assess responses that could be directly related to steelhead health condition during out-migration. These biomarkers were used on juvenile steelhead captured in the Snake River, a tributary of the Columbia River, in Washington, USA, and were applied on gill and anterior head kidney tissue to assess immune system responses, pathogen-defense (NRAMP, Mx, CXC), general
stress (HSP70), metal-binding (metallothionein-A), and xenobiotic metabolism (Cyp1a1) utilizing quantitative polymerase chain reaction (PCR) technology. Upon capture, fish were ranked according to visual external physical conditions into good, fair, poor,
and bad categories; gills and kidney tissues were then dissected and preserved for gene analyses. Transcription responses were tissue-specific for gill and anterior head kidney with less significant responses in gill tissue than in kidney. Significant differences between the condition ranks were attributed to NRAMP, MX, CXC, and Cyp1a1 responses. Gene profiling correlated gene
expression with pathogen presence, and results indicated that gene profiling can be a useful tool for identifying specific pathogen types responsible for disease. Principal component analysis (PCA) further correlated these responses with specific health condition categories, strongly differentiating good, poor, and bad condition ranks. We conclude that molecular profiling is an
informative and useful tool that could be applied to indicate and monitor numerous population-level parameters of management interest.

 

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5 Year Summary

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Status

Published
October (4th Quarter/Autumn) 2012

Unit Authors

Oregon Cooperative Fish and Wildlife Research Unit Cooperators

  1. Oregon Department of Fish and Wildlife
  2. Oregon State University
  3. U.S. Fish and Wildlife Service
  4. U.S. Geological Survey
  5. Wildlife Management Institute